PAG 2023
January 13–18, 2023
San Diego, California
We look forward to seeing you at PAG 2023! Join us to find out how new technology from PacBio can help fuel discovery in plant + animal research.
Complete the form on this page to confirm your attendance at our workshop, request a meeting with us at PAG, or to receive a recording of our talks, posters, and other content we’ll present at the conference.
Not able to attend? Not to worry! Sign up anyway for exclusive, on demand access to our scientific program.
In the meantime, see below and refer back to this page for more on our workshop, talks, and other details about PacBio at PAG 2023.
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Monday, January 16, 2023 12:50 - 3:00 PM PST
Town and Country Hotel, San Diego, CA
Jonas Korlach, PhD
CSO, PacBio
Introductory remarks
Gina Zastrow-Hayes
Genomics Technology Manager,
Corteva Agriscience
Ultra-high throughput workflows for agrigenomic applications
Brad Shaffer, PhD
Professor, UCLA, Director, California Conservation Genomics Project
Conserving California’s biodiversity with genomics
Daniel Portik, PhD
Senior Bioinformatics Scientist, PacBio
More and better: a new era for metagenome assembly
Sarah Carey, PhD
Post-Doctoral Fellow, HudsonAlpha Institute for Biotechnology
Exploring the multiple evolutions of sex chromosomes in plants
Authors: Jacob Brandenburg, Aaron Wenger, Christine Lambert, Heather Ferrao
Jacob Brandenburg, Senior Specialist, Segment Marketing, PacBio
Long-read whole genome sequencing (WGS) for plants and animals has allowed scientists to tackle the assembly of genomes ranging in size, ploidy, and repeat content. HiFi WGS enables complete, accurate, and contiguous assemblies of even the most complex genomes. As a result, HiFi WGS has been adopted by many biodiversity genomics consortia and agriculture researchers to assemble thousands of genomes. While HiFi WGS simplifies the bioinformatics of genome assembly, library preparation remains a common challenge, especially for scientists sequencing organisms which yield low quantities of DNA or have very large genomes that require multiple libraries for a single de novo assembly. We describe a new workflow using the PacBio SMRTbell Prep Kit 3.0 that is amenable to automation while significantly reducing the time (by 50%), cost, and DNA quantity (by 40%) necessary to prepare a sequencing library. We applied the workflow to rice and fly samples and demonstrate that the assembly quality matches that of the previous, more time-consuming library prep workflows. This new workflow offers a more efficient solution for all WGS applications, regardless of organism size or complexity.
Authors: Greg Concepcion, Michelle Vierra
Greg Concepcion, Staff Engineer, Bioinformatics, PacBio
With the increasing prevalence of high-quality assembled genomes, access to the pangenomes of species is now an achievable possibility for researchers with all research budgets. However, the tools to visualize, analyze, and extract actionable information from these pangenome datasets are still in their infancy. Using a large plant pangenome dataset, we evaluated the pangenome graph builder pipeline for building a pangenome graph of the species and use these evaluations to provide basic recommendations for how to best manipulate and visualize these pangenomic datasets with other downstream tools including the vg toolkit.
Monday, October XX X:00 pm PST
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Presenter name, title, credentials, Presenter name, title, credentials
Presenter name, title, credentials, Presenter name, title, credentials
Monday, October XX X:00 pm PST
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Lorem, ipsum dolor sit amet consectetur adipisicing elit. Eveniet quasi corporis, ut consectetur optio aut quas tempore, rerum ipsum vel eaque voluptates magnam a molestiae voluptatibus veniam quos architecto asperiores? Lorem ipsum dolor sit amet consectetur adipisicing elit. A quod deleniti, tempora reprehenderit, vel consequatur odit eveniet distinctio culpa libero ipsam? Ducimus quos amet laboriosam ut perferendis eos similique cum. Lorem ipsum dolor sit amet consectetur, adipisicing elit.
Presenter name, title, credentials, Presenter name, title, credentials
Presenter name, title, credentials, Presenter name, title, credentials
| # | Title | First Author | Time |
| PE0048 | Ultra-high throughput multi-omic analysis for agrigenomics on PacBio Revio | J. Burke | 10:00–11:30 AM |
| PE0684 | Maximizing MAGs from long-read metagenomic assemblies: a new post-assembly pipeline with circular-aware binning | J. Wilkinson | 10:00–11:30 AM |
| PO0049 | With Nanobind HMW DNA extraction, PacBio provides a complete end-to-end workflow for high quality genomes from a diversity of samples | M. Kim | 3:00–4:30 PM |
| PO0063 | Isoform resolution for single-cell transcriptomics with highly accurate long-read sequencing | G. Young | 3:00–4:30 PM |
| # | Title | First Author | Date |
| 1062 | A high-resolution panel for uncovering repeat expansions that cause ataxias | Y. Tsai | Febraury 28, 2022 |
Stop by our booth to chat with PacBio staff, get all your questions answered, and demo our new Revio and Onso sequencing systems live!
Show hours:
Interested in getting a sequencing project started? Stop by our service providers at PAG to talk about project design and next steps.
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Booth 211 |
Booth 406 |
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Booth 503 |
Booth 523 |
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Booth 319 |
Booth 434 |