Asia Pacific
HiFi Sequencing Experience Tour
Asia Pacific
Seoul - 20 September 2022 | Daejeon - 27 September 2022
Seoul - 2:00 - 6:00 pm | Daejeon - 2:00 - 5:00 pm
Seoul –Seoul National University, Cancer Research Institute, Lee Kun-Hee Auditorium (2F) | Daejeon –Chungnam National University, College of Natural Science, Gamminho Hall
Join us from 20 and 27 September 2022 in Seoul and Daejeon, with Korea being the third stop of the PacBio Asia Pacific HiFi Sequencing Experience Tour, to find out how HiFi sequencing is helping scientists find accurate and actionable genome-wide insights for the most important scientific discoveries.
There will be presentations by industry-leading scientists and the opportunity for peer-to-peer discussions and networking during this event. Whether you have a single sample or a multi-genome project, complete and accurate long-read sequencing is designed to support your desire to make an impact.
We look forward to meeting you.
Highlights include:
SEOUL - 20 SEPTEMBER 2022
Seoul National University, Cancer Research Institute, Lee Kun-Hee Auditorium (2F)
| TIME | TOPIC |
| 13:30 | Registrations |
| 14:00 | Welcome & Introductions |
| 14:10 | PacBio Company Overview |
| 14:20 | HiFi: Targeted Sequencing for Human Genetics |
| 14:40 | Microbial Metagenome Updates |
| 15:00 | De novo WGS of Antimicrobial-resistant Bacteria based on Long-read Technology |
| 15:30 | Meta Epigenetics: Discovering Marin e DNA Methylation Patterns beyond Metagenomics |
| 16:00 | Coffee Break |
| 16:20 | HiFi Sequencing for Epigenetics & Rare Disease |
| 16:40 | Full-length RNA transcripts & Single-cell RNA isoform analysis for cancer |
| 17:00 | Structural Variants near Human Telomeric Regions and unknown Isoform Detection using HiFi sequencing |
| 17:30 | PacBio HiFi sequencing: Global Genomics Initiatives |
| 17:40 | Event ends |
DAEJEON - 27 SEPTEMBER 2022
Chungnam National University, College of Natural Science, Gamminho Hall
| TIME | TOPIC |
| 13:30 | Registrations |
| 14:00 | Welcome & Introductions |
| 14:10 | PacBio Company Overview |
| 14:20 | HiFi: Targeted Sequencing for Human Genetics |
| 14:40 | Microbial Metagenome Updates |
| 15:00 | De novo WGS of Antimicrobial-resistant Bacteria based on Long-read Technology |
| 15:30 | Meta Epigenetics: Discovering Marin e DNA Methylation Patterns beyond Metagenomics |
| 16:00 | Coffee Break |
| 16:20 | HiFi Sequencing for Epigenetics & Rare Disease |
| 16:40 | Full-length RNA transcripts & Single-cell RNA isoform analysis for cancer |
| 17:00 | Structural Variants near Human Telomeric Regions and unknown Isoform Detection using HiFi sequencing |
| 17:30 | PacBio HiFi sequencing: Global Genomics Initiatives |
| 17:40 | Event ends |
REGISTER NOW
Senior Manager, PGx & HLA Market Development
PacBio
Director of Marketing, Asia Pacific
PacBio
Director, Human Genomics Market Development, Asia Pacific
PacBio
Sr. Bioiformatics Scientist, Field Application,
Asia Pacific
PacBio
Jeong Seok Hoon, MD, PhD
Department of Laboratory Medicine, Gangnam Severance Hospital, Yonsei University College of Medicine
De novo WGS of antimicrobial-resistant bacteria based on long-read technology:
De novo WGS using long-read technology is useful in the analysis of antimicrobial-resistant bacteria. In silico analysis of the WGS enables us to identify epidemiological information of host bacterial cells, such as sequence type, and cgMLST is a powerful tool in comparing epidemiological traits of bacteria. WGS also confers various information about location (plasmid or chromosome) and copy number of antimicrobial resistance determinants and structure of antimicrobial resistance determinant-carrying transposon or operon. In this lecture, I will show the results of WGS for CTX-M-type ESBL-producing E. coli and K. pneumoniae.
Kim Jun, PhD
Research Institute of Basic Sciences, Seoul National University
Structural variants near human telomeric regions and unknown isoform detection using HiFi sequencing:
HiFi 시퀀싱은 99.9% 이상의 높은 정확도를 보이는 20 kb 이상의 DNA와 RNA 정보를생산함으로써 기존에 시퀀싱 기법으로는 확인이 어려웠던 영역의 정보까지도 제공해주고 있다. 텔로미어와 그 인근 지역은 그 대표적인 예로, 반복서열이 밀집돼 있어GRCh38 (hg38)까지는 해당 지역의 정확한 정보를 알지 못해 N으로 채워진 상황이었다. 최근 발표된 CHM13 유전체지도는 이런 한계를 극복하고자 HiFi 시퀀싱을 비롯한long-read sequencing을 도입해 작성됐고, 그 결과 최초로 모든 텔로미어와 그 인근 지역을 확인한 인간 유전체 지도가 탄생할 수 있었다. 우리는 이런 HiFi 시퀀싱 기법의 장점을 살려 한국인 3인의 유전체 지도를 고품질로 작성했으며, 이를 기반으로 유전체 전반에 존재하는 50 bp 이상의 구조 변이(structural variant)가 기존 시퀀싱 기법에 비해 10배 가까이 정확하게 확보된다는 것을 확인할 수 있었다. 그뿐만 아니라 텔로미어 인근지역에서는 100 kb에 이르는 거대한 구조 변이가 나타남을 확인하였으며, HiFi 데이터의 높은 정확도를 바탕으로 이런 구조 변이가 생겨날 수 있었던 메커니즘을 추정할 수있었다. 또한 HiFi 시퀀싱은 기존에 밝혀지지 않았던 유전자동형(isoform)을 정확하게분석해낼 수 있는 강력한 방법론을 제공해주고 있다. 뼈 이식에 사용되는 골반뼈의 유전자동형 분석 결과를 공유함으로써 가능한 연구에 대해 소개하고자 한다.
Seong Hoon Je, PhD
Macrogen Inc.
Meta-Epigenetics: Discovering Marine DNA Methylation Patterns Beyond Metagenomics
Ma Junyeong, PhD Candidate
Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University
Assembly of complete prokaryotic genomes for human gut microbiome using HiFi sequencing
Despite advances in culturomics technology, most human gut prokaryotic species remain uncultured. Therefore, conventional cataloging of microbial genomes, based on isolation of clonal genomic DNA followed by sequencing and assembly, may not be applicable to all human gut commensals. De novo metagenomic assembly using human fecal sequencing samples has proven useful in reconstructing the genomes of gut species including uncultured taxa. However, these metagenome-assembled genomes (MAGs) are generally discontinuous because of conserved, repetitive, and mobile sequences. Long-read metagenomic sequencing using Oxford nanopore technology (ONT) has enabled the assembly of circularized complete MAGs (cMAGs), albeit with low nucleotide accuracy. More recently, PacBio high-accuracy long-read (HiFi) sequencing has been applied for the analysis of complex microbiomes. HiFi repetitive sequencing of a circularized SMRTbell library calls reads by consensus, substantially improving nucleotide accuracy while maintaining long read length. Moreover, specialized assemblers for HiFi metagenomic assembly enable the highly accurate reconstruction of cMAGs. In the present study, we report 102 cMAGs obtained by HiFi metagenomic sequencing of five human fecal samples, whose initial circular contigs were selected for complete prokaryotic genomes using our bioinformatics workflow. Nucleotide accuracy of the final cMAGs was similar to that of Illumina sequencing. The cMAGs could exceed 6 Mbp and included complete genomes of diverse taxa, including entirely uncultured RF39 and TANB77 orders, whose genomes are mostly under-characterized. Moreover, cMAGs revealed that regions hard to assemble by short-read sequencing comprised mostly genomic islands and rRNAs. HiFi metagenomic sequencing will facilitate cataloging accurate and complete genomes of microbiota, including uncultured species
Interested in getting a sequencing project started? Contact MDxK today
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