Unbiased characterization of metagenome composition and function using HiFi sequencing on the PacBio Sequel II System
 

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Date:      Thursday, October 10, 2019
Time:
     08:30 a.m.  New Delhi
     11:00 a.m.  Beijing, Kuala Lumpur, Singapore
     12:00 p.m.  Seoul, Tokyo
     02:00 p.m.  Melbourne

 

Guest Speaker:

Recent work comparing metagenomic sequencing methods indicates that a comprehensive picture of the taxonomic and functional diversity of complex communities will be difficult to achieve with NGS technology alone. While the low cost of prior technology enables high sequencing depth, the greater contiguity and low GC bias of long-read assemblies has advantages for gene finding. The introduction of the Sequel II System and SMRT Sequencing improvements have enabled a new, higher throughput, assembly-optional data type uniquely suited to metagenome characterization: HiFi reads. HiFi reads combine high accuracy with read lengths up to 15 kb, eliminating the need for assembly for most microbiome applications, including gene discovery and metabolic pathway reconstruction. The median read lengths and accuracy of HiFi reads extends beyond the assembly quality metrics afforded by prior technology, enabling more efficient and economical recovery of intact genes and operons while omitting the laborious and resource intensive assembly step.  In addition, no data is discarded because it cannot be assembled: every HiFi read yields 5-8 predicted genes. Finally, the high accuracy of HiFi data makes it compatible with standard gene prediction tools developed for data enabled by prior technology.


We look forward to your participation as you learn about SMRT Sequencing!
 

Meredith Ashby, Ph.D.
Director of Market Strategy, Cancer & Microbial Genomics

PacBio




 

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