Small Bodies, Big Genomes: Overcoming Large DNA Input
Requirements for Long-Read Genome Assembly
For Research Use Only. Not for use in diagnostics procedures.
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A high-quality reference genome is an essential tool in plant and animal genomics. However, relatively high DNA input requirements (≥5 µg for a standard library) have previously placed PacBio genome assemblies out of reach for many projects on small, non-inbred organisms that have limited DNA content.
In this webinar we will present the low DNA input workflow, from DNA requirements through library preparation and sequencing, for generation of high-quality genome assemblies of small-bodied organisms. In addition, we will highlight the use of this workflow on the North American ice worm (Mesenchytraeus solifugus), which has a genome size of ~1.6 Gb and lives its entire life cycle in glacier ice, creating a unique opportunity to look at adaptation to harsh environments.
Understand requirements for use of the low DNA input workflow for genome assembly
Learn how the low DNA input workflow enabled sequencing of the large genome of the small-bodied ice worm
Gain new insights into the ice worm genome and its adaptations to life in glacier ice
|Date:||Thursday, September 12, 2019|
|Time:||9:30 a.m. PDT
12:30 p.m. EDT
5:30 p.m. BST
University of Delaware Sequencing and Genotyping Center
Manager, Plant and Animal Sciences
School of Biological Sciences, Washington State University