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LIGHTNING TALKS: FAST-PACED UPDATES FROM OUR USER BASE
Welcome and Introduction
Jonas Korlach, Ph.D., Chief Scientific Officer, PacBio
The Trials and Tribulations of High Quality Human Genome Assembly
Tina Graves-Lindsay, M.S., Group Leader for Reference Genomes, McDonnell Genome Institute (MGI), Washington University School of Medicine in St. Louis
FEMTO Pulse QC Checkpoints Optimize and Accelerate Large Insert SMRTbell Library Preparations for PacBio
Bruce Kingham, M.S., Director, DNA Sequencing & Genotyping Center, Delaware Biotechnology Institute, University of Delaware
FALCON-Phase: Phased Diploid Assemblies through Integration of PacBio and Hi-C Data
Zev Kronenberg, Ph.D., Senior Computational Biologist
Development, Validation, and Applications of a High-fidelity, Species-specific, Eubacterial Pan-domain Microbiome Assay on the PacBio System
Garth D. Ehrlich, Ph.D., Professor of Microbiology and Immunology, and Otolaryngology-head and Neck Surgery, Drexel University College of Medicine
Structural Variant Calling in SMRT Link 6.0 (powered by pbsv)
Jonas Korlach, Ph.D., Chief Scientific Officer, PacBio
Calling all variants: fast, accurate, population-scale structural variant analysis
Roberto Lleras, Bioinformatic Field Application Scientist, PacBio
Low-input Workflow for PacBio De Novo Genome Assemblies
Jonas Korlach, Ph.D., Chief Scientific Officer, PacBio
Catching Large DNA in the SageHLS
Chris Boles, Ph.D., Chief Scientific Officer
Application of Genome Assembly in Bovinae Species
Timothy Smith, Ph.D., Molecular Geneticist, USMARC, USDA-ARS
Future Developments in SMRT Sequencing
Jonas Korlach, Ph.D., Chief Scientific Officer, PacBio
Iso-Seq as a Service: Pro Tips for Starting from RNA
Katherine Munson, Research Technician, Department of Genome Sciences, University of Washington
SMRT Multiplexing to Sequence Tough Repeats in Plant Pathogenic Bacteria
Jonathan Jacobs, Ph.D., Assistant Professor, Department of Plant Pathology, The Ohio State University
A Promoter Crystal Ball: How to Use Low-Coverage PacBio Data to Find Promoters in Non-Model Insects
Marcé D. Lorenzen, Ph.D., Associate Professor, Department of Entomology and Plant Pathology, North Carolina State University
Microbial Multiplexing for Fun and Profit: A Full-Workflow Case Study on a Collection of Diverse Isolates
Ben Auch, Innovation Lab, Genomics Center, University of Minnesota
The Long and Short of It: PacBio Amplicon Sequencing Applications
Dave Corney, Ph.D., Senior Scientist, Next-Generation Sequencing, GENEWIZ
Please note that not all speakers have given permission to share their presentations. Those that have are listed below.
Breakout Rotation 1:
SMRT Sequencing Project Success: Start to Finish
Breakout Rotation 2:
Evolving SMRT Sequencing Applications
Trinucleotide Repeat Diseases: CRISPR Cas9 PacBio no PCR Sequencing
Ross Aleff, Principal Research Technologist, Mayo Clinic
Breakout Rotation 3:
The Why, What, and How of the Iso-Seq Method: Using Full-Length Transcriptome Sequencing to Annotate Genomes and Solve Diseases
BREAKOUT ROTATIONS