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Thank you for joining us at PAG XXV in San Diego, California and furthering your understanding of how Single Molecule, Real-Time (SMRT) Sequencing can help advance your research by delivering long continuous reads, high consensus accuracy, uniform coverage, and simultaneous epigenetics characterization.
Below in the various sections, you will find links to the videos, presentations, and posters presented at the conference.
We also invite you to check out a comprehensive list of SMRT Sequencing publications, literature and resources, by visiting us at Additionally, here is our complete list of certified sequencing service providers.

SMRT Sequencing for Complete Genomes







View the slides of each individual presentation by clicking the presentation titles below.


Toward the Gapless Assembly of Complete Vertebrate Genomes
Sergey Koren, Ph.D., National Institutes of Health
From Contigs to Chromosomes
Ben Rosen, Ph.D., United States Department of Agriculture
Graphical Fragment Assembly GFA 2.0
Shaun Jackman, University of British Columbia
Characterizing Swine Transcriptomes by Combined Iso-Seq and RNA-Seq for Annotating the Emerging Long Read-based Reference Genomes
Haibo Liu, Iowa State University
A Glimpse into Size Selection Free Iso-Seq
Richard Kuo, Roslin Institute, University of Edinburgh
Hummingbird Iso-seq Analysis: A Bird’s Eye View of an Extreme Avian Metabolism
Rachael Workman, Johns Hopkins University
PacBio’s Unique Data, Analysis Software and Applications: SMRT Link v4.0
Aaron Klammer, Ph.D., PacBio
Phased Diploid Assembly Using Falcon Unzip
Greg Concepcion, Ph.D., PacBio

View each individual video presentation by clicking the presentation titles below.

Hosted by:

Workshop Welcome and Introduction
Jonas Korlach, Ph.D., Chief Scientific Officer, PacBio




View the slides of each individual presentation by clicking the presentation titles below.

Monday, January 16, 4:00 – 5:00 p.m.
Don’t count in the dark – Illuminate your transcriptome with the PacBio Iso-Seq Method
Making sense of RNA-seq data got you feeling down? Learn how to sequence full-length transcripts, no assembly required. Literally thousands of new genes and isoforms have been discovered with the Iso-Seq Method, but who’s counting?
Tuesday, January 17, 10:00 – 11:00 a.m.
Impress your mom – Join the 10(!) Mb Contig N50 Club with PacBio ultra-long libraries
Tired of working all day with your tiny contigs and nothing interesting to write home about? Join us for tips and tricks to achieve the highest quality genomic library preps for the most contiguous assemblies. Be the kid Mom can brag about!
Tuesday, January 17, 3:00 – 4:00 p.m.
Finish strong – Take that final hurdle to publication with PacBio SMRT (and easy) Analysis
Sequencing complete and feeling accomplished? Congrats! Let’s talk assembly. Join us to learn more about tools available from PacBio for easy data analysis – it’s all that’s standing in the way between you and a great new publication!

Please download the PacBio Program Flyer to discover the more than 115 presentations and posters featuring SMRT Sequencing to decipher complex plant and animal genomes. View PacBio posters by clicking the poster titles below.
Phased Diploid Genome Assembly with Single-Molecule Real-Time Sequencing
Greg Concepcion, Ph.D, PacBio
A High-Quality Genome Assembly of SMRT Sequences Reveals Long-Range Haplotype Structure in the Diploid Mosquito Aedes aegypti
Sarah Kingan, Ph.D., PacBio
Best Practices for Whole Genome Sequencing Using the Sequel System 
Nick Sisneros, PacBio
Full-Length cDNA Sequencing on the PacBio Sequel Platform
Tyson Clark, Ph.D., PacBio
Profiling Populations and Diversity of Gene Pools with Highly Accurate Single Molecule Reads
Cheryl Heiner, PacBio

Using Iso-Seq to Fill in Your A_no_tion
Richard Kuo, Bioinformatician, Roslin Institute, University of Edinburgh


De Novo Sequencing of the Koala Genome
Rebecca Johnson, Ph.D., Director, Australian Museum Research Institute, Science and Learning Division

Genome Assembly and Molecular Genetics of the Dengue, Yellow Fever, and Zika Vector Aedes Aegypti
Ben Matthews, Ph.D., Research Associate, Laboratory of Neurogenetics and Behavior, HHMI/Rockefeller University

Introducing 5 New High-quality PacBio Genome Assemblies for Rice to Help Solve the 10-Billion People Question
Rod A. Wing, Ph.D., Antle Professor, School of Plant Sciences, Director, Arizona Genomics Institute, University of Arizona, and AXA Chair, Genetics & Biotechnology Division, International Rice Research Institute