Beyond being an integral component of cellular development and homeostatic maintenance, RNA splicing is implicated in a wide range of pathologies with hallmark isoforms being linked to cardiovascular, neurological, and immunological diseases. While RNA-sequencing has dramatically accelerated our understanding of biology, quantitation and discovery of full-length RNA isoforms resulting from alternative splicing remain poorly resolved. This is due to the inability of existing sequencing platforms to scalably sequence full-length mRNAs – short-read approaches are unable to span most successive splice sites while long-read approaches are constrained by limited throughput.
In session 3 of our PacBio ASHG 2021 workshop, Aziz Al’Khafaji, Ph.D. (Broad Institute) talks about a new method called Multiplexed Arrays sequencing (MAS-seq), which maximizes the sequencing potential of isoforms on the PacBio platform.
Registering on this page grants you access to all four speaker sessions, which will air sequentially from 10:00–11:20 am PDT. You may choose to join us for the entire workshop, or simply drop in to the session(s) that interest you most.
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