Pacific Biosciences Workshop:
Whole Human Genome SMRT® Sequencing Reveals Uncharacterized Structural Variations Providing a Path to More Informed Diagnostic Testing

Eric Schadt, Ph.D.
Director, Icahn Institute for Genomics and Multiscale Biology at Mount Sinai School of Medicine

Friday, February 22, 3:20 p.m. – 3:40 p.m.
Islands Ballroom

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Thursday, February 21

Deep Sequencing of Immune Repertoires During Bovine Development and in Response to Respiratory Pathogen Challenge
- Tim Smith, Ph.D., U.S. Meat Animal Research Center

8:30 p.m. – 8:50 p.m., Islands Ballroom

Spatiotemporal Dynamics of the Epitranscriptome Reveal Novel Mechanisms of RNA Regulation
- Christopher Mason, Ph.D., Cornell Medical College

8:30 p.m. – 8:50 p.m., Salon G through J

Friday, February 22

Assembling Crop Genomes with Single Molecule Sequencing
- Michael Schatz, Ph.D., Cold Spring Harbor Laboratory

9:10 p.m. – 9:30 p.m., Salon G through J

Saturday, February 23

Increased Read Length and Sequence Quality with Pacific Biosciences MagBead Loading System and a New DNA Polymerase
- Eric Antoniou, Ph.D., Cold Spring Harbor Laboratory

9:30 a.m. – 9:55 a.m., Islands Ballroom

Automated, Non-Hybrid De Novo Genome Assemblies and Epigenomes of Bacterial Pathogens
- Jonas Korlach, Ph.D., Pacific Biosciences

11:35 a.m. – 12:00 p.m., Islands Ballroom

Thursday, February 21, 1:00 p.m. - 2:30 p.m.

#74: Optimization of Procedures of Genomic DNA Preparation in Diverse Organisms for Single Molecule, Real-Time Sequencing
 - Tomoko Shibata et al., National Institute of Basic Biology

#80: Integration of NGS Desktop Sequencers to Build a Global Genomic Network for Pathogen Trace-back and Outbreak Detection: Error Rates are not the Sole Criteria for Determining Applications for Instrumentation
 - Marc Allard et al., U.S. Food & Drug Administration

#99: Application of SMRT® Genome Sequencing to Reveal the Methylomes of Bacteria Associated with Respiratory Disease Outbreaks in Beef Cattle
 - Gregory Harhay et al., USDA-ARS-USMARC

#114: Closing Gaps in Finished Genomes using Long Reads and Local BAC Assembly
 - Todd Michael, Monsanto Company

#185: Advances in Sequence Consensus and Clustering Algorithm for Effective De Novo Assembly and Haplotyping Applications with SMRT® Sequencing
 - Jason Chin et al., Pacific Biosciences

#201: Assembling PacBio® Continuous Long Reads without Using Other Types of Reads
 - Takamasa Imai et al., The University of Tokyo

#210: Fast and Scalable Error Correction of SMRT Sequencing Human Genome Reads with Illumina Reads
 - Shoichiro Oishi et al., University of Tokyo

#260: Direct Sequencing of Small Genomes on the PacBio RS without Library Preparation
 - Paul Coupland et al., Wellcome Trust Sanger Institute

#277: Evaluating Genome Sequencing and Assembly Strategies for Diverse Microbial Pathogens
 - Xinyue Liu et al., University of Maryland, Institute for Genome Sciences

#300: Towards Rapid and Cost-effective Whole Exome Sequencing for Clinical Diagnosis and Personalized Medicine
 - Mark Wang, Baylor College of Medicine

#333: Full Length cDNA Sequencing on the PacBio® RS
 - Jason Underwood et al., Pacific Biosciences

Join us for some California-style beach volleyball!
Gatorade and snacks provided.

7 a.m. on Thursday, Friday, and possibly Saturday (weather permitting)
Where: At the sand courts outside the Marriott Hotel

Or feel free to come cheer us on!!!

Program Presentations


Friday, February 22, 4:30 p.m.– 6:00 p.m

#41: Single Molecule, Real-Time Sequencing for Base Modification Detection in Eukaryotic Organisms

 - Khai Luong et al., Pacific Biosciences

#51: Estimating Unmethylated DNA Blocks in Vertebrates by SMRT Sequencing
 - Yuta Suzuki et al., The University of Tokyo

#73: Phasing Variants in Poplar Trees using a Hybrid of Short and Long Read Technologies
 - Wendy Schackwitz et al., Lawrence Berkeley National Lab - JGI

#94: The Use of Long Sequence Reads to Ameliorate Microbial Genome Assemblies
 - Ken Dewar et al., McGill Universtiy

#96: The ABRF-NGS: a Five Platform, Cross-Site, Cross-Protocol Examination of RNA Sequencing
 - George Grills, Cornell University

#100: Pacific Biosystems SMRT® Technology as a Confirmatory Methodology for the Verification of DNA Sequence Variants found by NGS
 - Ed Highsmith, Jr. et al., Mayo Clinic

#117: SMRT® Sequencing for High-Resolution Typing and Phasing of HLA Alleles
 - Swati Ranade et al., Pacific Biosciences

#140: Human MUC5AC Genome Sequence:  Filling the Reference Gap by De Novo Assembly of PacBio Long Sequencing Reads
 - Hong Dang et al., UNC-Chapel Hill

#209: De Novo Assembly of Genomes Using Large Insert Pacific Biosciences® SMRT Sequencing Reads
 - Joel Martin et al., Lawrence Berkeley National Lab - JGI

#291: Characterizing and Measuring Bias in Sequence Data
 - Michael Ross et al., Broad Institute of MIT and Harvard

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Early Riser?
Evening Party

Join us for an evening of cocktails, hors d'oeuvres, and great conversation.

When: Friday, February 22, 9:30 p.m. - Midnight
Where: Lanai Suite #193

We look forward to hosting you!